PBS pH7.4
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 1:200 | Hu, Ms, Rt |
CD27 is a transmembrane protein that belongs to the tumor necrosis factor receptor superfamily (TNFRSF) and is primarily expressed on T cells, B cells, and natural killer (NK) cells. It activates NF-κB and JNK signaling pathways by binding to its ligand, CD70, thereby promoting the activation, proliferation, and differentiation of T cells. CD27 plays a crucial role in immune regulation, acting as an important co-stimulatory receptor for T and B cells, and is involved in modulating immune responses and the formation of memory cells. Additionally, CD27 is being studied as a potential target for anti-tumor immunotherapy.
Flow cytometric analysis of human peripheral blood cells labelling CD27 antibody at 1/200 (1 μg) dilution/ (Right panel) compared with a Amenian hamster IgG Isotype Control / (left panel). Goat Anti-Amenian hamster IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Brilliant Violet 421™ antibody at separately.
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling CD27 antibody at 1/200 (1 μg) dilution/ (Right panel) compared with a Amenian hamster IgG Isotype Control / (left panel). Goat Anti-Amenian hamster IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Brilliant Violet 421™ antibody at separately.
Flow cytometric analysis of SD Rat splenocytes labelling CD27 antibody at 1/200 dilution (1 μg) / (Red) compared with a Amenian hamster IgG (Black) Isotype Control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Amenian hamster IgG Alexa Fluor® 488 was used as the secondary antibody.
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