PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 1:500 | Hu |
CD69 is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily. It is a classical early marker of lymphocyte activation, appearing rapidly on the cell surface after stimulation. Structurally, CD69 is a disulfide-linked homodimer protein with two differentially glycosylated subunits (28–32 kDa), each consisting of an extracellular C-type lectin domain connected to a single-spanning transmembrane region followed by a short cytoplasmic tail. It is broadly expressed on the surfaces of most hematopoietic lineages, including activated T and B lymphocytes, natural killer (NK) cells, murine macrophages, neutrophils, and eosinophils, and is constitutively expressed on human monocytes, platelets, epidermal Langerhans cells, and bone-marrow myeloid precursors. CD69 plays various regulatory roles in immune responses, such as promoting tissue residency, regulating Th17/Treg cell differentiation, and contributing to the exhaustion of resident memory T cells, especially in the tumor microenvironment. Its ligands include galectin-1, calprotectin (S100A8/S100A9 complex), myosin light chains 9, 12a, and 12b (Myl9/12), and oxidized low-density lipoprotein (ox-LDL).
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) treated 48 hours with 10 μg/mL Phytohemagglutinin (PHA), labeling CD69 at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
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