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Biotin Rat Anti-Mouse I-A/I-E Antibody (S-R512)

Biotin Rat Anti-Mouse I-A/I-E Antibody (S-R512)

货号: S0B5243
价格: 300
规格: 100T
介绍: -
其他: -
产品规格
  • 宿主来源

    Rat
  • 抗原名称

    I-A/I-E
  • 分子别名

    H-2 class II histocompatibility antigen, I-A beta chain; H2-Eb1
  • 细胞定位

    Membrane
  • Accession

    P18468, Q3U060
  • 克隆号

    S-R512
  • 抗体类型

    Rat mAb
  • 抗体同种型

    IgG2a,k
  • 应用

    FCM
  • 反应种属 ?

    Ms
  • 阳性样本

    C57BL/6 mouse splenocytes
  • 纯化方式

    Protein G
  • 浓度

    20 μg/ml
  • 标记

    Biotin
  • 性状

    Liquid
  • 缓冲体系

    PBS, 1% BSA, 0.3% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
稀释度
应用 稀释度 推荐种属
FCM 5μl per million cells in 100μl volume Ms
背景介绍
  • I-A/I-E proteins are part of the Major Histocompatibility Complex (MHC) class II molecules, which are heterodimeric transmembrane glycoproteins expressed on antigen-presenting cells such as dendritic cells, macrophages, and B cells. These proteins are encoded by four genes located in the mouse MHC and are composed of α and β subunits. The I-A and I-E molecules are highly polymorphic and can form hybrid molecules in heterozygous individuals, contributing to the diversity of antigen presentation. They play a crucial role in presenting peptide antigens to CD4+ helper T cells, initiating adaptive immune responses. Additionally, MHC class II molecules can trigger intracellular signaling pathways in antigen-presenting cells, influencing their proliferation, maturation, and apoptosis.

  • 流式分析

    • Flow cytometric analysis of Mouse l-A/l-E expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Pacific Blue™ Rat Anti-Mouse CD45R/B220 Antibody and either Biotin Isotype Control (Left panel) or SDT Biotin Rat Anti-Mouse I-A/I-E Antibody (Right panel) at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.