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ABCA1 Recombinant Rabbit mAb (S-1887-25)

ABCA1 Recombinant Rabbit mAb (S-1887-25)

货号: S0B1471
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    ABCA1
  • 分子别名

    Phospholipid-transporting ATPase ABCA1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1 (ABC-1; ATP-binding cassette 1); Cholesterol efflux regulatory protein; ABC1; CERP
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cell membrane, Endosome
  • Accession

    O95477
  • 克隆号

    S-1887-25
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    WB
  • 反应种属 ?

    Hu, Ms, Rt
  • 阳性样本

    HepG2, U-87 MG, mouse liver, rat brain
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu, Ms, Rt
背景介绍
  • ABCA1 (ATP Binding Cassette Subfamily A Member 1) is a protein encoded by the ABCA1 gene, located on chromosome 9q31.1. It is a member of the ATP-binding cassette (ABC) transporter family, which uses energy from ATP hydrolysis to transport various substrates across cell membranes. ABCA1 specifically facilitates the efflux of cholesterol and phospholipids from cells to extracellular acceptors such as apolipoprotein A-I (APOA1), playing a crucial role in the formation of high-density lipoprotein (HDL) particles. This process is essential for reverse cholesterol transport (RCT), which helps remove excess cholesterol from tissues and prevents atherosclerosis. Mutations in the ABCA1 gene can lead to Tangier disease, a rare genetic disorder characterized by extremely low levels of HDL cholesterol and cholesterol accumulation in tissues.

  • 免疫印迹

    • WB result of ABCA1 Recombinant Rabbit mAb
      Primary antibody: ABCA1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HCT 116 whole cell unboiled RIPA lysate 20 µg
      Lane 2: untreated HepG2 whole cell unboiled RIPA lysate 20 µg
      Lane 3: HepG2 treated with 20 μM T0901317 for 16 hours whole cell unboiled RIPA lysate 20 µg
      Lane 4: U-87 MG whole cell unboiled RIPA lysate 20 µg
      Negative control: HCT 116 whole cell unboiled RIPA lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 254 kDa
      Observed MW: 300 kDa

    • WB result of ABCA1 Recombinant Rabbit mAb
      Primary antibody: ABCA1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse liver unboiled RIPA lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 254 kDa
      Observed MW: 300 kDa
      This blot was developed with high sensitivity substrate

    • WB result of ABCA1 Recombinant Rabbit mAb
      Primary antibody: ABCA1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat brain unboiled RIPA lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 254 kDa
      Observed MW: 290 kDa
      This blot was developed with high sensitivity substrate