PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:500-1:1000 | Hu |
CD300a protein is a member of the CD300 glycoprotein family, which are type I transmembrane proteins expressed on the surface of various leukocytes. It has an IgV-like extracellular domain and a long cytoplasmic tail containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which are crucial for its inhibitory signaling. CD300a is expressed in natural killer (NK) cells, subsets of T and B cells, monocytes, dendritic cells, granulocytes, and mast cells. It plays an inhibitory role in immune responses by regulating various functions such as inhibiting Ca++ mobilization in response to receptor stimulation, suppressing NK cell-mediated cytotoxicity, B-cell proliferation, and mast cell activation. Additionally, CD300a mediates immune tolerance to dying cells by binding to phosphatidylserine and phosphatidylethanolamine. Its expression and function are regulated by various cytokines and stimuli, and it has potential implications in allergic diseases and other immune-related conditions.
WB result of CD300a Rabbit pAb
Primary antibody: CD300a Rabbit pAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Lane 3: U-937 whole cell lysate 20 µg
Lane 4: HL-60 whole cell lysate 20 µg
Negative control: 293T whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 40-100 kDa
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