PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
Phospho-YAP (Ser127) is a phosphorylated form of the Yes-associated protein (YAP), which is a key transcriptional coactivator in the Hippo signaling pathway. YAP regulates various cellular processes, including cell proliferation, survival, differentiation, and organ size. The phosphorylation of YAP at serine 127 by kinases such as MST1/2 and LATS1/2 leads to its inactivation and cytoplasmic retention. When dephosphorylated, YAP can translocate to the nucleus and promote gene transcription. For example, androgen exposure can suppress the inhibitory phospho-Ser127 modification on YAP, resulting in increased YAP nuclear abundance and activity. This regulation is crucial for understanding the role of YAP in diseases like cancer.
WB result of Phospho-YAP (Ser127) Recombinant Rabbit mAb
Primary antibody: Phospho-YAP (Ser127) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 85 kDa
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