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Human IFN-γ OneStep OptiQuant ELISA Kit

Human IFN-γ OneStep OptiQuant ELISA Kit

货号: S0C3040
价格: 3200
规格: 1x96T
介绍: -
其他: -
产品规格
  • 抗原名称

    IFN-γ
  • 免疫原

    Recombinant Protein
  • 抗体类型

    Recombinant mAb
  • 反应种属 ?

    Hu
  • 纯化方式

    Protein A
  • 储存条件

    2 to 8 °C as supplied.

  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}]
试剂盒参数
    • Precision

      Intra-assay: 4.0%;
      Inter-assay: 4.5%
    • Sample type

      Cell culture supernatant, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      2.577pg/ml
    • Range

      15.63 pg/mL – 1000 pg/mL
    • Recovery

      Cell culture supernatant: 93%
      Serum: 109%
      Plasma: 111%
    • Assay time

      45 minutes
    • Species reactivity

      Human
    • NIBSC convert

      The dose response curve of the International Standard (87/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (87/586) units, use the following equation: NIBSC (87/586) approximate value (IU/mL) = 0.02 x OneStep Human IFNγ value (pg/mL).

背景介绍
  • Interferon-gamma (IFN-γ) is a dimeric cytokine central to innate and adaptive immunity, primarily produced by activated T cells and natural killer (NK) cells. Structurally, IFN-γ exists as a homodimer, with each subunit comprising six α-helical domains stabilized by intra- and interchain disulfide bonds. It signals through a heterodimeric receptor complex composed of IFNGR1 and IFNGR2, initiating JAK-STAT pathway activation. Post-translational glycosylation at asparagine residues (e.g., Asn25 and Asn97 in humans) enhances its stability and modulates receptor interaction dynamics. Immune Regulatory Roles and Therapeutic Cross-Talk of IFN-γ: Interferon-gamma (IFN-γ) orchestrates multifaceted immune responses, exerting critical antiviral and antitumor defense by inducing cellular antiviral states and enhancing tumor immunosurveillance through upregulation of MHC class I/II expression. It activates macrophages to bolster phagocytosis, reactive oxygen species production, and pathogen clearance, while polarizing CD4+ T cells into Th1 subsets to amplify cell-mediated immunity. Despite its protective roles, IFN-γ exhibits a dual nature in inflammation, where excessive levels contribute to autoimmune disorders (e.g., rheumatoid arthritis, multiple sclerosis) and chronic inflammatory pathologies. Therapeutically, IFN-γ synergizes with proinflammatory cytokines like TNF-α and IL-12 to amplify immune activation, while counteracting the immunosuppressive effects of IL-4 and IL-10, highlighting its pivotal yet complex role in balancing immune defense and inflammatory regulation. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 45 minutes, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IFN-γ quantification in time-sensitive or resource-limited settings.

  • ELISA

    • Standard curve
      Example of Human IFN-γ standard curve in Assay Diluent #10.

    • Linearity
      The concentrations of IFN-γ were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows:  human PBMC cells were stimulated with 10ug/ml PHA for 6days (0.83%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 81.32 ng/mL in stimulated human PBMC supernatant.

    • Linearity
      To assess the linearity of the assay, three samples were spiked with high concentrations of Human IFN-γ in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Protocol Diagram