2 to 8 °C as supplied.
Granzyme B (GrB) is a serine protease pivotal in cytotoxic immune responses, mediating apoptosis and immune surveillance. Structurally, GrB is synthesized as a 32 kDa inactive pro-enzyme (zymogen) and activated through proteolytic cleavage. Its active form features a conserved catalytic triad (His57, Asp102, Ser195) essential for enzymatic activity. Post-translational modifications, including N-linked glycosylation, enhance its stability and regulate intracellular trafficking. GrB functions by binding to mannose-6-phosphate receptors (MPRs) for endosomal uptake into target cells, where it synergizes with perforin to induce cell death. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 60 minutes, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable Granzyme B quantification in time-sensitive or resource-limited settings.
Standard curve
Example of Human Granzyme B standard curve in Assay Diluent #10.
Linearity
The concentrations of Granzyme B were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 6 days (0.625%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 696.65 ng/mL in stimulated human PBMC supernatant.
Linearity
#2 sample is undiluted samples are as follows: human PBMC cells culture for 6 days (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1.659 ng/mL in unstimulated human PBMC supernatant.
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