PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms |
| ICC | 1:500 | Hu |
Phospho-LATS1/2(Ser909/872) refers to the phosphorylated forms of the mammalian Hippo signaling pathway kinases LATS1 (Large Tumor Suppressor 1) and LATS2 at specific serine residues (Ser909 in LATS1 and Ser872 in LATS2). As core components of this pathway, LATS1/2 are activated through phosphorylation by upstream kinases MST1/2, which subsequently phosphorylate downstream effector proteins YAP/TAZ to regulate their nucleocytoplasmic shuttling, degradation, and biological functions, thereby suppressing cell proliferation and promoting apoptosis. Phosphorylation at Ser909/872 is likely a critical step in LATS1/2 activation, potentially modulating their kinase activity, stability, or interactions with regulatory proteins. This phosphorylation event is commonly used as a biomarker of Hippo pathway activity, detectable via specific antibodies (e.g., in Western blotting), and is studied in contexts such as cancer and organ development due to the pathway's strong association with tumorigenesis and metastasis. Notably, species-specific variations or splice isoforms may alter residue numbering, and functional validation often involves mutagenesis experiments (e.g., phosphomimetic glutamic acid substitutions or non-phosphorylatable alanine mutations) to assess impacts on pathway regulation.
WB result of Phospho-LATS1/2(Ser909/872) Recombinant Rabbit mAb
Primary antibody: Phospho-LATS1/2(Ser909/872) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 127, 120 kDa
Observed MW: 140, 120 kDa
WB result of Phospho-LATS1/2(Ser909/872) Recombinant Rabbit mAb
Primary antibody: Phospho-LATS1/2(Ser909/872) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: serum-starved NIH/3T3 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 127, 120 kDa
Observed MW: 130, 115 kDa
Dot blot result of Phospho-LATS1/2(Ser909/872) Recombinant Rabbit mAb
Lane 1: LATS1/2(Ser909/872) phospho peptide
Lane 2: LATS1/2(Ser909/872) unmodified peptide
Primary antibody: Phospho-LATS1/2(Ser909/872) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
ICC analysis of HeLa cells treated with 100 ng/ml Calyculin A for 30 minutes (top panel) and untreated HeLa cells (below panel). Anti- Phospho-LATS1/2(Ser909/872) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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