PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
The ASGR2 protein (Asialoglycoprotein Receptor Subtype 2) is a type II transmembrane receptor primarily expressed on the surface of hepatocytes and belongs to the C-type lectin superfamily. It forms hetero-oligomeric complexes with ASGR1, mediating the endocytosis and lysosomal degradation of desialylated glycoproteins in the bloodstream by recognizing terminal galactose or N-acetylgalactosamine residues. This process plays a critical role in maintaining glycoprotein homeostasis and regulating cholesterol metabolism. ASGR2 exhibits liver-specific high expression, featuring a calcium-dependent carbohydrate-recognition domain (CRD) in its extracellular region. Recent studies have identified the ASGR signaling pathway's involvement in lipid metabolism disorders, liver fibrosis, and hepatocellular carcinoma progression. Targeted therapies (e.g., ASGR1/2 inhibitors) show potential for cholesterol-lowering treatments, possibly by modulating the PCSK9/LDLR pathway. Additionally, ASGR2 is utilized in designing liver-targeted drug delivery systems, leveraging its efficient internalization capacity to enhance liver-specific accumulation of therapeutic molecules.
WB result of ASGR2 Recombinant Rabbit mAb
Primary antibody: ASGR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 50 kDa
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-ASGR2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cervix cancer. Anti-ASGR2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-ASGR2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of ASGR2 in tumor tissue.
Expression of ASGR2 in human tissue.
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