PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
CD98 (Cluster of Differentiation 98) is a heterodimeric transmembrane glycoprotein composed of a heavy chain (CD98hc, encoded by SLC3A2) and a light chain (e.g., LAT1, encoded by SLC7A5), connected by disulfide bonds. It is widely expressed in various cell types, including immune cells, epithelial cells, and tumor cells. Functionally, CD98 is primarily involved in amino acid transport, particularly the transmembrane movement of neutral amino acids, which are essential for cell proliferation and metabolism. Additionally, CD98 interacts with integrin β1 to regulate cell adhesion, migration, and signaling. In the immune system, it plays a role in lymphocyte proliferation and activation, as well as in inflammatory responses. In pathological contexts, CD98 is highly expressed in various cancers and is associated with tumor invasion, metastasis, and drug resistance. For example, in extranodal natural killer/T-cell lymphoma (ENKTL), CD98 enhances chemoresistance via exosome-mediated mechanisms. High expression of CD98 in solid tumors is also linked to poor prognosis and may serve as a biomarker for tumor progression and treatment response. Given its critical role in tumor metabolism and signaling, CD98 has emerged as a potential therapeutic target for cancer.
WB result of CD98/SLC3A2 Recombinant Rabbit mAb
Primary antibody: CD98/SLC3A2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: Molt-4 whole cell lysate 20 µg
Lane 5: U-2 OS whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 70-120 kDa
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CD98/SLC3A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD98/SLC3A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-CD98/SLC3A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-CD98/SLC3A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-CD98/SLC3A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-CD98/SLC3A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of CD98/SLC3A2 in tumor tissue.
Expression of CD98/SLC3A2 in human tissue.
您现在的位置：