PBS, 1% BSA, 0.3% Proclin 300
应用 | 稀释度 | 推荐种属 |
---|---|---|
FCM | 5 μl per million cells in 100μl volume | Ms |
CD23, also known as the low-affinity IgE receptor (FcεRII), is a 45 kDa type II integral membrane glycoprotein that plays a crucial role in regulating IgE production and B-cell differentiation. It is expressed on various immune cells, including mature B cells, monocytes, eosinophils, follicular dendritic cells, and platelets. CD23 exists in two isoforms, CD23a and CD23b, which differ in their amino acid sequences and functions. The molecule can also be cleaved into a soluble form (sCD23) that retains IgE-binding capabilities.
Flow cytometric analysis of Mouse CD23 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD19 Antibody and either Rat IgG2a, κ Isotype Control (Left panel) or SDT PE Rat Anti-Mouse CD23 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.