PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
4E-BP1 (eukaryotic initiation factor 4E-binding protein 1) is a key regulator of protein translation and plays a crucial role in controlling the assembly of the eIF4F complex, which is essential for cap-dependent translation initiation. It is the most widely expressed and well-characterized member of the 4E-BP family in mammalian cells. When active, 4E-BP1 binds to eIF4E, preventing its interaction with eIF4G and thereby inhibiting the formation of the eIF4F complex, which is necessary for the translation of many oncogenic mRNAs with structured 5′-UTRs. This mechanism is critical in suppressing tumor development, as hyperactive eIF4E-dependent translation contributes to the upregulation of pro-tumorigenic genes in cancer cells. The activity of 4E-BP1 is regulated by various signaling pathways, including the PI3K/AKT/mTOR pathway, which can lead to its phosphorylation and inactivation, allowing eIF4E to promote translation. Reactivating 4E-BP1 through mTOR inhibition or other mechanisms has been shown to have tumor-suppressive effects.
WB result of 4E-BP1 Recombinant Rabbit mAb
Primary antibody: 4E-BP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Lane 5: HEK-293 whole cell lysate 20 µg
Lane 6: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 13 kDa
Observed MW: 18 kDa
IHC shows positive staining in paraffin-embedded human cervix cancer. Anti-4E-BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-4E-BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-4E-BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-4E-BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate cancer. Anti-4E-BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in HeLa cells. Anti- 4E-BP1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression of 4E-BP1 in tumor tissue.
Expression of 4E-BP1 in human tissue.
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