PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
Phospho-Acetyl-CoA Carboxylase (Ser79) is a phosphorylated form of Acetyl-CoA Carboxylase (ACC), a key enzyme in lipid metabolism that catalyzes the conversion of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. When phosphorylated at Ser79, ACC is inhibited, preventing the production of malonyl-CoA and thus suppressing fatty acid synthesis. This phosphorylation is primarily regulated by AMP-activated protein kinase (AMPK), which is activated under low energy conditions, such as fasting, to promote fatty acid oxidation and conserve energy. ACC exists in two isoforms, ACC1 and ACC2, with ACC1 mainly involved in fatty acid synthesis and ACC2 regulating fatty acid oxidation by inhibiting carnitine palmitoyltransferase I (CPT-I) in mitochondria. Phospho-ACC (Ser79) antibodies are widely used to detect the phosphorylated state of ACC in various tissues and species, including human, mouse, and rat.
WB result of Phospho-Acetyl-CoA Carboxylase (Ser79) Recombinant Rabbit mAb
Primary antibody: Phospho-Acetyl-CoA Carboxylase (Ser79) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HEK-293 whole cell lysate 20 µg
Lane 2: HEK-293 treated with 0.5 µM oligomycin for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 276 kDa
Observed MW: 270 kDa
Dot blot result of Phospho-Acetyl-CoA Carboxylase (Ser79) Recombinant Rabbit mAb
Lane 1: Acetyl-CoA Carboxylase (Ser79) phospho peptide
Lane 2: Acetyl-CoA Carboxylase (Ser79) unmodified peptide
Primary antibody: Phospho-Acetyl-CoA Carboxylase (Ser79) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
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