PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Phospho-EGFR (Tyr1173) is a phosphorylated form of the epidermal growth factor receptor (EGFR), a 170 kDa transmembrane receptor tyrosine kinase that plays a crucial role in cell signaling. When EGFR binds to its ligands, such as EGF, it undergoes dimerization and autophosphorylation, activating multiple downstream signaling pathways. Phosphorylation at Tyr1173 is particularly important as it provides a docking site for proteins like the tyrosine phosphatase SHP-1, which can modulate EGFR activity and downstream signaling. This site is also involved in the MAP kinase signaling pathway, which regulates cell growth, survival, and transformation. Abnormal regulation of EGFR, including phosphorylation at Tyr1173, is often associated with various cancers, making it a key target for therapeutic interventions.
WB result of Phospho-EGFR (Tyr1173) Recombinant Rabbit mAb
Primary antibody: Phospho-EGFR (Tyr1173) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated A431 whole cell lysate 20 µg
Lane 2: A431 treated with 200 ng/ml EGF for 10 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 134 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
Dot blot result of Phospho-EGFR (Tyr1173) Recombinant Rabbit mAb
Lane 1: EGFR (Tyr1173) phospho peptide
Lane 2: EGFR (Tyr1173) unmodified peptide
Primary antibody: Phospho-EGFR (Tyr1173) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
ICC analysis of A431 cells treated with EGF (200 ng/mL for 10 min) (top panel) and A431 cells untreated with EGF (200 ng/mL for 10 min) (below panel). Anti- Phospho-EGFR (Tyr1173) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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