PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
Phospho-PERK (Thr980) is a phosphorylated form of the protein kinase-like endoplasmic reticulum kinase (PERK), a type I transmembrane protein located in the endoplasmic reticulum (ER). It plays a crucial role in the unfolded protein response (UPR) by activating itself through oligomerization and autophosphorylation at threonine 980 (Thr980) in response to ER stress and the accumulation of misfolded proteins. This phosphorylation enhances PERK's activity, allowing it to phosphorylate eukaryotic translation initiation factor 2 alpha (eIF2α), which leads to the attenuation of translation initiation and global protein synthesis. This process helps protect cells from the toxic effects of misfolded proteins and maintains cellular homeostasis. Additionally, the activation of PERK is associated with cell cycle arrest due to the loss of cyclin D1 and the regulation of genes involved in metabolism, redox status, and apoptosis.
WB result of Phospho-PERK (Thr980) Recombinant Rabbit mAb
Primary antibody: Phospho-PERK (Thr980) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 1 µM thapsigargin for 20 minutes and 100 nM Calyculin A for 15 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 125 kDa
Observed MW: 180 kDa
Dot blot result of Phospho-PERK (Thr980) Recombinant Rabbit mAb
Lane 1: mouse PERK (Thr980) phospho peptide
lane 2: human PERK (Thr982) phospho peptide
lane 3: mouse PERK (Thr980) unmodified peptide
lane 4: human PERK (Thr980) unmodified peptide
Primary antibody: Phospho-PERK (Thr980) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
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