PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:200 | Hu |
| ICC | 1:500 | Hu |
Promyelocytic leukemia protein (PML), also known as TRIM19, is a multifunctional protein belonging to the TRIM family and is best known for its role in the formation and stability of PML nuclear bodies (PML-NBs) within the cell nucleus. These nuclear bodies are membraneless organelles that serve as hubs for various cellular processes, including transcriptional regulation, DNA damage repair, apoptosis, and antiviral defense. PML itself is a 70 kDa protein with an N-terminal RBCC motif (RING, B-box, and coiled-coil domains) that facilitates its oligomerization and interaction with over 160 other proteins. The protein is also subject to post-translational modifications such as SUMOylation, phosphorylation, and ubiquitination, which regulate its function and localization. PML has been implicated as a tumor suppressor, with its downregulation observed in various cancers, highlighting its importance in maintaining cellular homeostasis and preventing carcinogenesis.
WB result of PML Recombinant Rabbit mAb
Primary antibody: PML Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HUVEC whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50-110 kDa
Observed MW: 45-130 kDa
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PML antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous carcinoma. Anti-PML antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate cancer. Anti-PML antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-PML antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in Jurkat cells. Anti- PML antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression of PML in tumor tissue.
Expression of PML in human tissue.
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