PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:500 | Hu |
BrdU (5-bromo-2'-deoxyuridine) is a thymidine analog that can be incorporated into newly synthesized DNA strands during DNA replication, used to detect cell proliferation. BrdU antibodies are monoclonal or polyclonal antibodies that specifically recognize BrdU-labeled DNA and are widely used in experiments such as immunohistochemistry and flow cytometry to visualize or quantitatively analyze proliferating cells.
ICC analysis of Hela cells treated with BrdU (10uM for 24 hours) (top panel) and untreated THP-1 cells (below panel). The cells were fixed with 100% methanol (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. The cells were then permeabilized with 0.1% PBS-Triton X-100. Anti- BrdU (FITC Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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