PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5μl per million cells in 100μl volume | Hu, Ms |
CD324, also known as E-cadherin or cadherin-1, is a calcium-dependent cell adhesion protein that plays a crucial role in maintaining epithelial tissue integrity and regulating cell proliferation, differentiation, and survival. It is a member of the cadherin superfamily and is widely expressed on the cell surface of most epithelial tissues. The extracellular domain of CD324 facilitates homophilic binding between adjacent cells, while its cytoplasmic domain interacts with the actin cytoskeleton through catenins. This protein is essential for epithelial cell cohesion and acts as an invasion suppressor, with its loss often indicating high tumor aggressiveness in cancer.
Flow cytometric analysis of CD324 expression on HT-29 cells. Cells from the HT-29 (Human colorectal adenocarcinoma epithelial cell, Right) or MDA-MB-231 (Human breast adenocarcinoma epithelial cell, Left) was stained with either Alexa Fluor® 647 Rat IgG1, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 647 Rat Anti-Mouse/Human CD324 Antibody (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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