PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
应用 | 稀释度 | 推荐种属 |
---|---|---|
FCM | 5μl per million cells in 100μl volume | Ms |
CD172a, also known as SIRP alpha (signal-regulatory protein alpha), is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed on cells of myeloid origin, including monocytes, macrophages, dendritic cells, and granulocytes, but not on T and B lymphocytes. SIRP alpha plays a crucial role in immune regulation by interacting with its ligand, CD47, which is widely expressed on various cell types, including cancer cells. This interaction transmits a "do not eat me" signal to prevent phagocytosis of healthy cells and is involved in processes like dendritic cell-mediated T cell activation and neutrophil migration. Additionally, SIRP alpha has regulatory effects on cellular responses induced by growth factors, insulin, and oncogenes, and it is implicated in both physiological and pathological processes, such as cancer progression.
Flow cytometric analysis of CD172a (SIRPα) expression on BALB/c mouse bone marrow. BALB/c mouse bone marrow was stained with Brilliant Violet 421™ Rat Anti-Mouse CD11b Antibody and either FITC Rat IgG1, κ Isotype Control (Left panel) or SDT FITC Rat Anti-Mouse CD172a (SIRPα) Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.