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Thioredoxin/TRX Recombinant Rabbit mAb (S-1862-26)

Thioredoxin/TRX Recombinant Rabbit mAb (S-1862-26)

货号: S0B1447
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    Thioredoxin/TRX
  • 分子别名

    Thioredoxin, mitochondrial; MTRX; Mt-Trx; Thioredoxin-2; TRX2; TXN2
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Mitochondrion
  • Accession

    Q99757
  • 克隆号

    S-1862-26
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    IHC-P, ICC, WB
  • 反应种属 ?

    Hu, Ms, Rt
  • 阳性样本

    HepG2, HeLa, Molt-4, Daudi, HL-60, Raji, Neruo-2a, C2C12
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu, Ms, Rt
IHC-P 1:250-1:1000 Hu, Ms, Rt
ICC 1:500 Hu
背景介绍
  • Thioredoxin (TRX) is a highly conserved redox-regulatory protein that plays a crucial role in maintaining cellular redox homeostasis and modulating various cellular processes. The TRX system, which includes TRX, thioredoxin reductase (TRXR), and NADPH, acts as an essential antioxidant defense mechanism against oxidative stress by reducing oxidized proteins and neutralizing reactive oxygen species (ROS). TRX exists in multiple isoforms, such as cytosolic TRX1, mitochondrial TRX2, and spermatozoa TRX, with TRX1 being the most prominent and widely studied. It functions through its redox-active site (CGPC), which can reduce disulfide bonds in target proteins and transfer electrons to peroxiredoxins to eliminate oxidative stress. TRX also regulates gene expression, DNA synthesis, cell proliferation, and apoptosis, and has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Additionally, TRX can be secreted into the extracellular space under oxidative stress, acting as a chemotactic factor for immune cells and serving as a marker of oxidative stress.

  • 免疫印迹

    • WB result of Thioredoxin/TRX Recombinant Rabbit mAb
      Primary antibody: Thioredoxin/TRX Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HepG2 whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: Molt-4 whole cell lysate 20 µg
      Lane 4: Daudi whole cell lysate 20 µg
      Lane 5: HL-60 whole cell lysate 20 µg
      Lane 6: Raji whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 18 kDa
      Observed MW: 13 kDa

    • WB result of Thioredoxin/TRX Recombinant Rabbit mAb
      Primary antibody: Thioredoxin/TRX Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Neuro-2a whole cell lysate 20 µg
      Lane 2: C2C12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 18 kDa
      Observed MW: 13 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti- Thioredoxin/TRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human liver. Anti- Thioredoxin/TRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach. Anti- Thioredoxin/TRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Thioredoxin/TRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti- Thioredoxin/TRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti- Thioredoxin/TRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney. Anti- Thioredoxin/TRX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat stomach. Anti- Thioredoxin/TRX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HepG2 cells. Anti- Thioredoxin / TRX antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Thioredoxin/TRX in human tissue.

    • Expression of Thioredoxin/TRX in mouse & rat tissue.

    • Expression of Thioredoxin/TRX in tumor tissue.