PBS pH7.4
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 1:2000 | Ms |
CD194, also known as CCR4 (C-C chemokine receptor type 4), is a G protein-coupled receptor with seven transmembrane domains. It is expressed on various immune cells, including CD4+ T cells (especially Th2 cells), regulatory T cells (Tregs), basophils, monocytes, and natural killer (NK) cells. CCR4 binds to chemokines such as CCL17 (TARC) and CCL22 (MDC), playing a crucial role in the recruitment of memory T cells and immune responses in conditions like atopic dermatitis and asthma. Additionally, CCR4 is involved in tumor biology, with high expression levels associated with tumor infiltration and prognosis in both hematological malignancies and solid tumors, making it a potential target for cancer immunotherapy.
WB result of ××× Rabbit mAb
Primary antibody: ××× Rabbit mAb at 1/××× dilution
Lane 1: ××× whole cell lysate 20 µg
Lane 2: ××× whole cell lysate 20 µg
Lane 3: ××× whole cell lysate 20 µg
Negative control: ××× whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: ××× kDa
Observed MW: ××× kDa
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse CD194 antibody at 1/2000 (0.1 μg) dilution/ (Right panel) compared with an Armenian hamster IgG Isotype Control / (Left panel). Goat Anti-Armenian hamster IgG Alexa Fluor® 488 was used as the secondary antibody. Cells were stained with CD3 - Alexa Fluor® 647 Antibody separately.
××× Rabbit mAb at 1/×× dilution (1 µg) immunoprecipitating ××× in 0.4 mg ××× whole cell lysate.
Western blot was performed on the immunoprecipitate using××× Rabbit mAb at 1/××× dilution.
Secondary antibody (HRP) for IP was used at 1/××× dilution.
Lane 1: ××× whole cell lysate 20 µg (Input)
Lane 2: ××× Rabbit mAb IP in ××× whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in ××× whole cell lysate
Predicted MW: ××× kDa
Observed MW: ××× kDa
IHC shows positive staining in paraffin-embedded human ×××. Anti-××× antibody was used at 1/××× dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IF shows positive staining in paraffin-embedded ×××. Anti-××× antibody was used at 1/××× dilution (magenta) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded ×××. Anti-××× antibody was used at 1/××× dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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