PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
| ICC | 1:500 | Hu |
PI3 Kinase p85 beta is a regulatory subunit of the phosphoinositide 3-kinase (PI3K) complex, which is crucial for generating 3-phosphorylated phosphoinositides that initiate a wide range of signaling pathways. Unlike its counterpart p85 alpha, p85 beta has both overlapping and unique functions. While p85 beta alone does not significantly impact B cell development, it can partially compensate for the loss of p85 alpha in B cell development and peripheral survival. Additionally, p85 beta has been shown to have distinct effects on apoptosis and may play a role in oncogenic transformation in certain cell types. Its presence is essential for maintaining proper signaling balance, as its deletion can lead to increased BCR-mediated proliferation and altered ERK phosphorylation.
WB result of PI3 Kinase p85 beta Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p85 beta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 85 kDa
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-PI3 Kinase p85 beta antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-PI3 Kinase p85 beta antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PI3 Kinase p85 beta antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-PI3 Kinase p85 beta antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in HeLa cells. Anti- PI3 Kinase p85 beta antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression of PI3 Kinase p85 beta in human tissue.
Expression of PI3 Kinase p85 beta in tumor tissue.
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