PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
Phospho-RIPK1 (Ser166) is a phosphorylated form of Receptor-Interacting Serine/Threonine-Protein Kinase 1 (RIPK1), a key regulator of TNF-mediated apoptosis, necroptosis, and inflammatory pathways. This protein consists of an N-terminal kinase domain, an intermediate domain containing the RIP homotypic interaction motif (RHIM), and a C-terminal death domain. When phosphorylated at Ser166, RIPK1 is involved in the formation of necrosis-inducing complexes and is considered a biomarker for the activation of RIPK1 kinase and necroptosis. This phosphorylation can be effectively inhibited by Nec-1, a necroptosis inhibitor.
WB result of Phospho-RIP (Ser166) Recombinant Rabbit mAb
Primary antibody: Phospho-RIP (Ser166) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
Lane 2: 293T transfected with RIP-Myc-His fusion protein, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 76 kDa
Observed MW: 85 kDa
Dot blot result of Phospho-RIP (Ser166) Recombinant Rabbit mAb
Lane 1: RIP (Ser166) phospho peptide 1
lane 2: RIP (Ser166) phospho peptide 2
lane 3: RIP (Ser166) unmodified peptide 1
lane 4: RIP (Ser166) unmodified peptide 2
Primary antibody: Phospho-RIP (Ser166) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
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