PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
Histone H3 is a core component of the nucleosome, the fundamental unit of chromatin, which plays a crucial role in organizing genomic DNA within the eukaryotic nucleus. The citrullination of Histone H3 at arginine residues R2, R8, and R17 is a specific post-translational modification catalyzed by peptidyl arginine deiminase 4 (PAD4). This modification prevents methylation of these residues by protein-arginine methyltransferase 4 (PRMT4) and is associated with transcriptional repression. Citrullinated Histone H3 is also a key component of neutrophil extracellular traps (NETs) and acts as an autoantigen, contributing to the production of anti-citrullinated protein antibodies in various diseases, including sepsis, multiple sclerosis, rheumatoid arthritis, and multiple myeloma.
WB result of Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb
Primary antibody: Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HEK-293 whole cell lysate 20 µg
Lane 2: HEK-293 transfected with PADI4 expression vector, then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Dot blot result of Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb
Lane 1: Histone H3 (citrulline R2+R8+R17) modified peptide
Lane 2: Histone H3 (citrulline R2) modified peptide
Lane 3: Histone H3 (citrulline R8) modified peptide
Lane 4: Histone H3 (citrulline R17) modified peptide
Lane 5: Histone H3 (citrulline R2+R8+R17) unmodified peptide
Primary antibody: Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
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