PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
Phospho-PKCδ (Thr505) refers to the phosphorylation of protein kinase C delta (PKCδ) at threonine residue 505, a modification that is crucial for its activation and function. PKCδ is a member of the protein kinase C (PKC) family, which are serine/threonine kinases involved in various cellular signaling pathways, including regulation of cell growth, apoptosis, and differentiation. The phosphorylation at Thr505 is particularly important for the kinase activity of PKCδ and its role in the G1 to S phase transition during cell cycle progression. This phosphorylation site is specific to PKCδ, and antibodies targeting Phospho-PKCδ (Thr505) do not cross-react with other PKC isoforms, making it a valuable marker for studying PKCδ's unique functions.
WB result of Phospho-PKCδ (Thr505) Recombinant Rabbit mAb
Primary antibody: Phospho-PKCδ (Thr505) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 treated with 0.05 µM PMA for 12 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDa
Dot blot result of Phospho-PKCδ (Thr505) Recombinant Rabbit mAb
Lane 1: PKC
δ
(Thr505) phospho peptide
Lane 2: PKC
δ
(Thr505) unmodified peptide
Primary antibody: Phospho-PKCδ (Thr505) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
您现在的位置：