PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
Transferrin Receptor 2 (TFR2) is a single-pass type II membrane protein primarily expressed in the liver and bone marrow, playing a crucial role in iron homeostasis. Unlike its more widely studied counterpart, Transferrin Receptor 1 (TFR1), TFR2 is specifically involved in regulating iron uptake and signaling. It interacts with transferrin, a blood plasma protein that transports iron, and helps modulate the expression of hepcidin, the master regulator of iron absorption and distribution in the body. Mutations in the TFR2 gene are associated with hereditary hemochromatosis type III, a condition characterized by excessive iron accumulation. Additionally, TFR2 is involved in erythropoiesis, as it coordinates erythroid iron availability with erythropoietin receptor sensitivity. Its therapeutic potential lies in treating iron-related disorders, such as hereditary hemochromatosis and anemia of chronic disease, by modulating iron absorption and distribution.
WB result of Transferrin Receptor 2/TFR2 Recombinant Rabbit mAb
Primary antibody: Transferrin Receptor 2/TFR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-87 MG whole cell lysate 20 µg
Lane 2: TF-1 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Negative control: U-87 MG whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 89 kDa
Observed MW: 100 kDa
IHC shows positive staining in paraffin-embedded human liver. Anti-Transferrin Receptor 2/TFR2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Transferrin Receptor 2/TFR2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of Transferrin Receptor 2/TFR2 in human tissue.
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