50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
12 months from date of receipt / reconstitution, -20 °C as supplied
应用 | 稀释度 |
---|---|
WB | 1:500-1:1000 |
ICC | 1:50-1:200 |
Ferritin Heavy Chain (FTH1) is a subunit of the iron storage protein Ferritin, primarily responsible for the oxidation and storage of iron ions to maintain cellular iron homeostasis and prevent iron-mediated oxidative damage. FTH1 possesses ferroxidase activity, enabling it to oxidize toxic ferrous iron (Fe²⁺) into stable ferric iron (Fe³⁺) and store it within the Ferritin protein shell. It plays a critical role in regulating iron metabolism, antioxidant stress, and cellular protection, with its expression modulated by iron levels, oxidative stress, and inflammatory signals. Dysfunction of FTH1 is associated with various diseases, including neurodegenerative disorders, cancer, and iron overload conditions. Additionally, FTH1 is involved in immune regulation and apoptosis processes.
WB result of Ferritin Heavy Chain Recombinant Rabbit mAb
Primary antibody: Ferritin Heavy Chain Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µ
Lane 2: CHO-K1 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 21 kDa
WB result of Ferritin Heavy Chain Recombinant Rabbit mAb
Primary antibody: Ferritin Heavy Chain Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 21 kDa
Immunocytochemistry analysis of Ferritin(green) in Hela using Ferritin antibody,and DAPI(blue).