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Scavenging Receptor SR-BI Recombinant Rabbit mAb (S-1736-15)

Scavenging Receptor SR-BI Recombinant Rabbit mAb (S-1736-15)

货号: S0B1412
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    Scavenging Receptor SR-BI
  • 分子别名

    Scavenger receptor class B member 1; SRB1; CD36 and LIMPII analogous 1 (CLA-1); CD36 antigen-like 1; Collagen type I receptor; thrombospondin receptor-like 1; SR-BI; CD36; CD36L1; CLA1; SCARB1
  • 免疫原

    Recombinant Protein
  • 细胞定位

    Cell membrane
  • Accession

    Q8WTV0
  • 克隆号

    S-1736-15
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    IHC-P, ICC, WB
  • 反应种属 ?

    Hu, Ms, Rt
  • 阳性样本

    HepG2, THP-1, NIH/3T3, rat liver
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000-1:5000 Hu, Ms, Rt
IHC-P 1:1000 Hu, Ms, Rt
ICC 1:500 Hu
背景介绍
  • Scavenger receptor class B type I (SR-BI) is a versatile and physiologically significant HDL receptor that plays a crucial role in cholesterol homeostasis and lipoprotein metabolism. It is a cell surface glycoprotein highly expressed in the liver, steroidogenic tissues, endothelium, and other tissues. SR-BI mediates the selective uptake of cholesterol esters from HDL particles into cells through hydrophobic channels without internalizing the entire lipoprotein, facilitating reverse cholesterol transport. This process helps remove excess cholesterol from peripheral tissues and promotes its excretion via bile acids, thus exerting anti-atherosclerotic effects. Additionally, SR-BI is involved in various biological functions, including regulation of inflammation, endothelial cell function, and lipid metabolism in macrophages. Its dysfunction or genetic mutations are associated with increased cardiovascular risk and altered HDL metabolism.

  • 免疫印迹

    • WB result of Scavenging Receptor SR-BI Recombinant Rabbit mAb
      Primary antibody: Scavenging Receptor SR-BI Recombinant Rabbit mAb at 1/5000 dilution
      Lane 1: HepG2 whole cell lysate 20 µg
      Lane 2: THP-1 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 61 kDa
      Observed MW: 70 kDa

    • WB result of Scavenging Receptor SR-BI Recombinant Rabbit mAb
      Primary antibody: Scavenging Receptor SR-BI Recombinant Rabbit mAb at 1/5000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 61 kDa
      Observed MW: 70 kDa

    • WB result of Scavenging Receptor SR-BI Recombinant Rabbit mAb
      Primary antibody: Scavenging Receptor SR-BI Recombinant Rabbit mAb at 1/5000 dilution
      Lane 1: rat liver lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 61 kDa
      Observed MW: 90 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human liver. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse liver. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat liver. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HepG2 cells. Anti-Scavenging Receptor SR-BI antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Scavenging Receptor SR-BI in tumor tissue.

    • Expression of Scavenging Receptor SR-BI in human tissue.

    • Expression of Scavenging Receptor SR-BI in mouse & rat tissue.