PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:250 | Hu, Ms |
Aldehyde dehydrogenase 1A1 (ALDH1A1) is an NADP-dependent enzyme belonging to the ALDH family, playing a crucial role in various physiological and toxicological processes. It is widely recognized as a marker for tumor-initiating cells and is involved in maintaining the stemness of tumor cells, promoting tumor angiogenesis and metastasis, and contributing to resistance against anticancer drugs. ALDH1A1 also lowers intracellular pH in breast cancer cells, suppressing antitumor immunity and fostering cancer progression. Recent studies have shown that ALDH1A1 upregulates ZBTB7B, a transcription factor that promotes glycolysis in tumor cells, leading to increased lactate production and immune evasion. This highlights ALDH1A1's potential as a therapeutic target for cancer immunotherapy.
WB result of ALDH1A1 Rabbit pAb
Primary antibody: ALDH1A1 Rabbit pAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 55 kDa
WB result of ALDH1A1 Rabbit pAb
Primary antibody: ALDH1A1 Rabbit pAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 50 kDa
IHC shows positive staining in paraffin-embedded human testis. Anti-ALDH1A1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ALDH1A1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ALDH1A1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-ALDH1A1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-ALDH1A1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of ALDH1A1 in tumor tissue.
Expression of ALDH1A1 in human tissue.
Expression of ALDH1A1 in mouse & rat tissue.
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