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Huntingtin Recombinant Rabbit mAb (S-1481-147)

Huntingtin Recombinant Rabbit mAb (S-1481-147)

货号: S0B1392
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Huntingtin

  • 分子别名

    Huntington disease protein (HD protein); HD; IT15; HTT

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus, Cytoplasm

  • Accession

    P42858

  • 克隆号

    S-1481-147

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICC, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, SH-SY5Y, Neuro-2a, mouse brain, PC-12, rat brain

  • 纯化方式

    Protein A

  • 浓度

    2 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu, Ms, Rt
ICC 1:2000 Hu
背景介绍

  • Huntingtin (HTT) is a large soluble protein composed of approximately 3144 amino acids, with a molecular weight of around 348 kDa. It is highly expressed in the central nervous system, particularly in neurons of the cerebral cortex and hippocampus. Huntingtin plays crucial roles in various cellular processes, including clathrin-mediated endocytosis, axonal transport, and synaptic signal transduction. It also helps protect neurons from apoptosis.

  • 免疫印迹

    • WB result of Huntingtin Recombinant Rabbit mAb
      Primary antibody: Huntingtin gamma Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: SH-SY5Y whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 348 kDa
      Observed MW: 348 kDa
      This blot was developed with high sensitivity substrate

    • WB result of Huntingtin Recombinant Rabbit mAb
      Primary antibody: Huntingtin gamma Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Neuro-2a whole cell lysate 20 µg
      Lane 2: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 348 kDa
      Observed MW: 348 kDa
      This blot was developed with high sensitivity substrate

    • WB result of Huntingtin Recombinant Rabbit mAb
      Primary antibody: Huntingtin gamma Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Lane 2: rat brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 348 kDa
      Observed MW: 348 kDa
      This blot was developed with high sensitivity substrate

  • 免疫细胞化学

    • ICC shows positive staining in SH-SY5Y cells. Anti- Huntingtin antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).