PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Ms |
IKK gamma, also known as NEMO (NF-κB essential modulator), is a crucial regulatory subunit of the IκB kinase (IKK) complex. The IKK complex, which also includes the catalytic subunits IKKα and IKKβ, plays a central role in activating the NF-κB signaling pathway. IKK gamma itself does not possess kinase activity, but it is essential for the assembly and activation of the IKK complex. When cells are stimulated by various factors such as cytokines, bacterial products, or viral infections, the IKK complex is activated, leading to the phosphorylation and degradation of IκB proteins. This process releases NF-κB dimers, allowing them to translocate to the nucleus and regulate the expression of numerous genes involved in inflammation, immune responses, and cell survival. Mutations in the gene encoding IKK gamma can lead to severe immunodeficiencies and developmental disorders, highlighting its critical role in maintaining immune function and cellular homeostasis.
WB result of IKK gamma Recombinant Rabbit mAb
Primary antibody: IKK gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 55 kDa
WB result of IKK gamma Recombinant Rabbit mAb
Primary antibody: IKK gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 55 kDa
WB result of IKK gamma Recombinant Rabbit mAb
Primary antibody: IKK gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 55 kDa
IHC shows positive staining in paraffin-embedded human testis. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in NIH/3T3 cells. Anti-IKK gamma antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression of IKK gamma in tumor tissue.
Expression of IKK gamma in human tissue.
Expression of IKK gamma in mouse & rat tissue.
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