R969-E2527(end)
MSPILGYWKIKGLVQPTRLL LEYLEEKYEE HLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIAYSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCLDAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSMRHSDSIS SLASEREYIT
SLDLSANELR DIDALSQKCC ISVHLEHLEK LELHQNALTS FPQQLCETLK SLTHLDLHSNKFTSFPSYLL KMSCIANLDV SRNDIGPSVV LDPTVKCPTL KQFNLSYNQL SFVPENLTDV
VEKLEQLILE GNKISGICSP LRLKELKILN LSKNHISSLS ENFLEACPKV ESFSARMNFLAAMPFLPPSM TILKLSQNKF SCIPEAILNL PHLRSLDMSS NDIQYLPGPA HWKSLNLREL
LFSHNQISIL DLSEKAYLWS RVEKLHLSHN KLKEIPPEIG CLENLTSLDV SYNLELRSFPNEMGKLSKIW DLPLDELHLN FDFKHIGCKA KDIIRFLQQR LKKAVPYNRM KLMIVGNTGS
GKTTLLQQLM KTKKSDLGMQ SATVGIDVKD WPIQIRDKRK RDLVLNVWDF AGREEFYSTHPHFMTQRALY LAVYDLSKGQ AEVDAMKPWL FNIKARASSS PVILVGTHLD VSDEKQRKAC
MSKITKELLN KRGFPAIRDY HFVNATEESD ALAKLRKTII NESLNFKIRD QLVVGQLIPDCYVELEKIIL SERKNVPIEF PVIDRKRLLQ LVRENQLQLD ENELPHAVHF LNESGVLLHF
QDPALQLSDL YFVEPKWLCK IMAQILTVKV EGCPKHPKGI ISRRDVEKFL SKKRKFPKNYMTQYFKLLEK FQIALPIGEE YLLVPSSLSD HRPVIELPHC ENSEIIIRLY EMPYFPMGFW
SRLINRLLEI SPYMLSGRER ALRPNRMYWR QGIYLNWSPE AYCLVGSEVL DNHPESFLKITVPSCRKGCI LLGQVVDHID SLMEEWFPGL LEIDICGEGE TLLKKWALYS FNDGEEHQKI
LLDDLMKKAE EGDLLVNPDQ PRLTIPISQI APDLILADLP RNIMLNNDEL EFEQAPEFLLGDGSFGSVYR AAYEGEEVAV KIFNKHTSLR LLRQELVVLC HLHHPSLISL LAAGIRPRML
VMELASKGSL DRLLQQDKAS LTRTLQHRIA LHVADGLRYL HSAMIIYRDL KPHNVLLFTLYPNAAIIAKI ADYGIAQYCC RMGIKTSEGT PGFRAPEVAR GNVIYNQQAD VYSFGLLLYD
ILTTGGRIVE GLKFPNEFDE LEIQGKLPDP VKEYGCAPWP MVEKLIKQCL KENPQERPTSAQVFDILNSA ELVCLTRRIL LPKNVIVECM VATHHNSRNA SIWLGCGHTD RGQLSFLDLN
TEGYTSEEVA DSRILCLALV HLPVEKESWI VSGTQSGTLL VINTEDGKKR HTLEKMTDSVTCLYCNSFSK QSKQKNFLLV GTADGKLAIF EDKTVKLKGA APLKILNIGN VSTPLMCLSE
STNSTERNVM WGGCGTKIFS FSNDFTIQKL IETRTSQLFS YAAFSDSNII TVVVDTALYIAKQNSPVVEV WDKKTEKLCG LIDCVHFLRE VTVKENKESK HKMSYSGRVK TLCLQKNTAL
WIGTGGGHIL LLDLSTRRLI RVIYNFCNSV RVMMTAQLGS LKNVMLVLGY NRKNTEGTQKQKEIQSCLTV WDINLPHEVQ NLEKHIEVRK ELAEKMRRTS VE
-80℃
LRRK2 G130517011 enzyme vs Initial velocity
The LRRK2 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the LRRK2 protein , ATP and substrate at 25℃ for 120 min, then adding ADP-Glo reagent and reading RLU with BMG.
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