Y99-G398，N-terminal GST tag
YVSRGGGPPPCEVASFQELRLEEVIGIGGFGKVYRGSWRGELVAVKAARQDPDEDISVTAESVRQEARLFAMLAHPNIIALKAVCLEEPNLCLVMEYAAGGPLSRALAGRRVPPHVLVNWAVQIARGMHYLHCEALVPVIHRDLKSNNILLLQPIESDDMEHKTLKITDFGLAREWHKTTQMSAAGTYAWMAPEVIKASTFSKGSDVWSFGVLLWELLTGEVPYRGIDCLAVAYGVAVNKLTLPIPSTCPEPFAQLMADCWAQDPHRRPDFASILQQLEALEAQVLREMPRDSFHSMQEG
59.8 kDa
50 mM Tris, 150 mM NaCl, 20 mM GSH, 1 mM DTT, 10% glycerol, 0.05% Brij-35, pH7.5
Stable for
12 months upon stored at -80℃ from the date of receipt. And avoid repeated
freeze-thaws cycles.
The MLK3 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the MLK3 protein , ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU with BMG.
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