M1-V672(end)
102577.0
-80℃
The PKCα activity was detected using HTRF technology. The reaction was performed by incubating the PKCα protein, ATP and substrate at 25℃ for 60 min, then adding beads at 25℃ for 60 min and reading ratio 665/620 with BMG.
M MW Standard
1 1 μg PKCα
2 3 μg PKCα