M1-A619(end)
96494.0
-80℃
The ZAP70 activity was detected using HTRF technology. The reaction was performed by incubating the ZAP70 protein, ATP and substrate at 25℃ for 60 min, then adding beads at 25℃ for 60 min and reading ratio 665/620 with BMG.
M MW Standard
1 1 μg ZAP70
2 3 μg ZAP70
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