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B7-H3/CD276 Fc Chimera Protein, Human

B7-H3/CD276 Fc Chimera Protein, Human

货号: UA010607
价格: 2000
规格: 100μg
介绍: -
其他: -
产品规格
  • 物种

    Human
  • 分子别名

    B7 homolog 3 (B7-H3), CD276, B7 homolog 3
  • Accession

    Q5ZPR3-2
  • 表达序列

    Leu29-Pro245, with C-terminal Human IgG1 Fc

  • 表达宿主

    HEK293
  • 分子量

    60-70kDa

  • 纯度

    >95% by SDS-PAGE
  • 内毒素含量

    <0.1EU/μg
  • 标记

    Unconjugated
  • 标签

    Human Fc Tag
  • 性状

    Lyophilized Powder
  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. A I Chapoval et al. B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production.Nat Immunol. 2001 Mar;2(3):269-74.

  • 稀释度

背景介绍
  • B7 homolog 3 (B7-H3, CD276) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 protein contains two extracellular Ig-like V-type domains and two IgG-like C2-type domains, a transmembrane domain, and a short intracellular domain. Early research examining the biological process of B7-H3 suggested that B7-H3 is a positive regulator of T cell response. Subsequent research studies indicated that B7-H3 is a negative regulator of T cell response, and that the protein inhibits T cell proliferation. One possibility is that B7-H3 interacts with two distinct sets of receptors, resulting in seemingly opposite biological outcomes. B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate. Expression of B7-H3 is seen in several cancer types, including prostate, breast, colon, lung, and gastric cancers, and in endothelial cells from tumor associated vasculature.

  • 流式分析

    • 2e5 of transient transfected anti-B7-H3 ScFv CAR-293 cells were stained with 0.01ug B7-H3/CD276 Fc Chimera Protein, Human , (Cat. No. UA010607) and Goat Anti-Human IgG, Fcγ Fragment Specific and unrelated protein (Fig. C and B and D), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor® 647 signal was used to evaluate the binding activity. 2e5 of transient transfected anti-B7-H3 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. F and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.

  • 电泳JSON

    • 1μg (R: reducing conditions, N: non-reducing conditions).

  • ELISA

    • Immobilized B7-H3/CD276 Fc Chimera Protein, Human (Cat. No. UA010607) at 2.0μg/mL (100μL/well) can bind Biotinylated Anti-Human B7-H3 (Enoblituzumab) with EC50 of 5.16-8.03ng/mL.