CRBN(M1-L442 end)
DDB1(M1-H1140 end)
N-terminal 6x His tag
53.7 KDa/126.9 KDa
50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
The CRBN/DDB1 activity was detected using HTRF technology. The reaction was performed by incubating the varying concentration of lenalidomide, CRBN/DDB1 protein, substrate and beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
M MW Standard
1 3 μg CRBN/DDB1
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