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CRBN GST&His Tag Protein, Human

CRBN GST&His Tag Protein, Human

货号: UA070005
价格: 1200
规格: 10μg
介绍: -
其他: -
产品规格

  • 物种

    Human

  • 分子别名

    CRBN, AD-006, MRT2, MRT2A

  • Accession

    Q96SW2

  • 表达序列

    Met1-Leu442, with N-terminal GST

    MSGSHHHHHHSSGMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLGHTGHRSGDDDDKMAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL

  • 表达宿主

    E.coli

  • 分子量

    76-78kDa (Reducing)

  • 纯度

    >90% by SDS-PAGE

  • 内毒素含量

    <1EU/μg

  • 标记

    Unconjugated

  • 标签

    GST Tag, His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 储存条件

    · 12 months from date of receipt, -20 to -70 °C as supplied. 
    · 6 months, -20 to -70 °C under sterile conditions after reconstitution.
    · 1 week, 2 to 8 °C under sterile conditions after reconstitution.  
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. lioua, A. , Kundu, P. , Lu, R. , Kumar, Y. , & Toro, L. . (2009). Large conductance calcium-activated potassium channels. Encyclopedia of Neuroence, 373-381.
    2. Higgins, J. J. , Pucilowska, J. ,  Lombardi, R. Q. , &  Rooney, J. P. . (2004). A mutation in a novel atp-dependent lon protease gene in a kindred with mild mental retardation. Neurology, 63(10), 1927-1931.

  • 稀释度

背景介绍

  • Cereblon (CRBN; a member of the ATP-dependent Lon protease) is an enzyme implicated in memory and learning. Interestingly, CRBN and KCa1.1 are both highly expressed in the same areas of the brain, including the hippocampus, which is known for its involvement in memory and learning. These two proteins bind directly to each other via the C-terminus of KCa1.1 in vitro, and their proximity in brain has been confirmed biochemically and by visualization of immunolabeled complexes in cultured hippocampal neurons. Ectopically expressed CRBN suppresses KCa1.1 surface expression by disrupting the formation of tetramers and therefore functional channels. The physiological consequences of the interaction between CRBN and KCa1.1 remain to be explored, although inherited mutations of the human cereblon gene (CRBN) or KCa1.1 lead to mental retardation disorders. And substrate recognition component of a DCX (DDB1-CUL4-X- Box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Regular degradation of Key regulatory proteins is necessary for Normal limb outgrowth and expression of the fibroblast growth factor FGF8.

  • 电泳JSON

    • 2μg (R: reducing conditions, N: non-reducing conditions).