E564-T1049
83259.0
50 mM Tris, pH7.4, 300 mM NaCl, 2 mM DTT, 10% glycerol
-80℃
The SOS1 activity was detected using HTRF technology. The reaction was performed by incubating the SOS1 protein, KRAS[G12D] and beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
M MW Standard
1 1 μg SOS1
2 3 μg SOS1