M1-D732(end)
85570.0
-80℃
The HSP90α activity was detected using HTRF technology. The reaction was performed by incubating the varying concentration of geldanamycin, HSP90α protein, substrate and beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
M MW Standard
1 1 μg HSP90α
2 3 μg HSP90α