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HB-EGF Protein, Human

HB-EGF Protein, Human

货号: UA040029
价格: 480
规格: 10μg
介绍: -
其他: -
产品规格
  • 物种

    Human
  • 分子别名

    Proheparin-binding EGF-like growth factor (HB-EGF)
  • Accession

    Q99075
  • 表达序列

    Asp63-Leu148
  • 表达宿主

    E.coli
  • 分子量

    13-15kDa (Reducing)
  • 纯度

    >95% by SDS-PAGE

  • 内毒素含量

    <1EU/μg
  • 标记

    Unconjugated
  • 标签

    No Tag
  • 性状

    Lyophilized Powder
  • 缓冲体系

    50mM Tris, 300mM NaCl, pH8.0
  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.
  • 稀释度

背景介绍
  • Proheparin-binding EGF-like growth factor (HB-EGF), also known as DTR, DTS and HEGFL, is a 13-15kDa member of the EGF family of mitogens. It is expressed in macrophages, monocytes, endothelial cells and muscle cells. HB-EGF signals through the EGF receptor to stimulate the proliferation of smooth muscle cells, epithelial cells and keratinocytes. Compared to EGF, HB-EGF binds to the EGF receptor with a higher affinity and has been shown to bemore mitogenic, likely due to its ability to bind to heparin and heparin sulfate proteoglycans. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. The transmembrane form of HB-EGF is the unique receptor for diptheria toxin and functions in juxtacrine signaling in cells. Both forms of HB-EGF participate in normal physiological processes and in pathological processes including tumor progression and metastasis, organ hyperplasia, and atherosclerotic disease. HB-EGF can bind two locations on cell surfaces, heparan sulfate proteoglycans and EGF-receptor effecting cell to cell interactions.

  • 生物活性JSON

    • Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells, the EC50 for this effect is less than 3ng/ml.
  • 电泳JSON

    • 1μg (R: reducing condition, N: non-reducing condition).
  • SPR

    • EGFR Fc Chimera, Human (Cat. No. UA010174) captured on Protein A Biosenor, can bind HB-EGF, Human (Cat. No. UA040029) with an affinity constant of 0.39 μM as determined in SPR assay.