Q9NZQ7, Phe19-Arg238, with C- hIgG1 Fc
FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNER
65-70 kDa (Reducing)
PBS, pH7.2, 5% trehalose
Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation .
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
细胞程式死亡-配体1(Programmed cell death 1 ligand 1，PD-L1)是大小为 40kDa人类体内表达的第一型跨膜蛋白，据信其在某些特殊情形(例如怀孕、组织移植、自体免疫疾病，以及诸如肝炎等某些疾病)下，免疫系统的抑制有关。正常情形下免疫系统会对聚集在淋巴结或脾脏的外来抗原产生反应，促进抗原特异性的细胞毒杀性T细胞(CD8+ T cell)增生。而细胞程序化死亡受体-1(PD-1)与细胞程式死亡-配体1(PD-L1)结合，可以传导抑制性的信号，减低淋巴结CD8+ T细胞的增生，而且PD-1还可以借此调节Bcl-2基因，控制淋巴结中抗原特异性T细胞的聚积。 本产品系由HEK293细胞株表达人源PD-L1，融合了human IgG1 Fc的重组蛋白，经多步纯化后过滤、添加5%海藻糖、分装冻干制成。
Immobilized PD-1 His Tag, Human (Cat. No. abs05023) at 1.5μg/mL (100μL/well) can bind PD-L1 Fc Chimera, Human(Cat. No. abs05024) with an EC50 of 0.142-0.174µg/mL.
Immobilized PD-1 His Tag, Cynomolgus (Cat. No. UA010311) at 2.0μg/mL (100μL/well) can bind PD-L1 Fc Chimera, Human (Cat. No. UA010003) with EC50 of 0.048-0.063μg/ml.
PD-L1 His Tag, Human (Cat. No.UA010001) captured on CM5 chip via anti-His antibody, can bind PD-1 Fc Chimera, Human (Cat. No. UA010002) with an affinity constant of 0.1836 μM as determined in a SPR assay (Biacore T200).
Loaded PD-L1 Fc Chimera, Human (Cat. No. UA010003) on Protein A Biosensor, can bind PD-1 His Tag, Human (Cat. No. UA010004) with an affinity constant of 1.6 μM as determined in BLI assay.
Protein A Chip captured PD-L1 Fc Chimera, Human (Cat. No. UA010003), can bind PD-1 His Tag, Cynomolgus (Cat. No. UA010311) with an affinity constant of 0.78 μM as determined in SPR assay.
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