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CD47 Fc Chimera Protein, Human

CD47 Fc Chimera Protein, Human

货号: UA010006
价格: 1300
规格: 25μg
介绍: -
其他: -
产品规格

  • 物种

    Human

  • 分子别名

    CD47 Fc Chimera protein ;antigen identified by monoclonal 1D8, Antigenic surface determinant protein OA3, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 antigen, CD47 glycoprotein, CD47 molecule, CD47, IAP, IAPintegrin associated protein, Integrin-associated protein, leukocyte surface antigen CD47, MER6, MER6integrin-associated signal transducer, OA3, Protein MER6, Rh-related antigen

  • Accession

    Q08722

  • 表达序列

    Gln19-Pro139, with C-terminal Human IgG Fc

  • 表达宿主

    HEK293

  • 分子量

    55~65 kDa(Reducing)

  • 纯度

    ＞95%, by SDS-PAGE under reducing conditions

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    Human Fc Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.2, 5% trehalose

  • 溶解方法

    Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation .

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 稀释度

背景介绍

  • CD47，也被称作整合素相关蛋白（Integrin-associated protein, IAP），是所有类型细胞表达的靶点中“marker of self”的典型代表。CD47是一个糖蛋白，具有免疫球蛋白可变的N端结构域、5个跨膜结构域和一个短的C端胞内尾，胞内尾部有四种可变不同剪接异构体，从而形成4个亚型。CD47是靶向肿瘤免疫治疗中发挥主要作用的免疫细胞类群，癌细胞表达的CD47与NK细胞表达的SIRP家族蛋白SIRPα以及SIRPγ相互作用，通过这样的方式保护癌细胞避免被吞噬消除。CD47在多种实体瘤细胞及恶性血液瘤细胞上呈现高表达，而且其表达水平与疾病进展呈正相关。

  • 生物活性JSON

    • Immobilized Human SIRP-α His tag (Cat. No. UA010009) at 1μg/mL (100μL/well) can bind CD47 Fc Chimera, Human(Cat. No. UA010006) with EC50 of 3.91-5.60ng/mL.

    • Immobilized SIRP-α/CD172a His Tag, Cynomolgus (Cat. No. UA010772) at 2.0μg/mL (100μL/well) can bind CD47 Fc Chimera, Human (Cat. No. UA010006) with EC50 of 1.34-1.91 ng/mL. 

  • 电泳JSON

    • CD47 Fc Chimera, Human, 2μg on SDS-PAGE under Non-reducing and reducing condition. The purity is greater than 95%.

  • SPR

    • Anti-His antibody Immobilized on CM5 Chip captured SIRP-α His Tag, Human (Cat. No. UA010009), can bind CD47 Fc Chimera, Human(Cat. No. UA010006) with an affinity constant of 4.828 nM as determined in SPR assay.

    • Protein A Chip captured CD47 Fc Chimera, Human (Cat. No. UA010006), can bind SIRP-α/CD172a His Tag, Cynomolgus(Cat. No. UA010772) with an affinity constant of 43.88nM as determined in SPR assay.