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FITC-Labeled EGFR Fc Chimera Protein, Human

FITC-Labeled EGFR Fc Chimera Protein, Human

货号: UA010380
价格: 3000
规格: 25μg
介绍: -
其他: -
产品规格

  • 物种

    Human

  • 分子别名

    EGFR, ERBB, ERRP, HER1, mENA, ERBB1

  • Accession

    P00533

  • 表达序列

    Leu25-Ser645, with C-terminal Human IgG1 Fc LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIEGRMDPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

  • 表达宿主

    HEK293

  • 分子量

    110-125kDa

  • 纯度

    >95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    FITC

  • 标签

    Human Fc Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4, 5% trehalose

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1、Schlessinger J. (2000) Cell signaling by receptor tyrosine kinases. Cell. 103(2): 211-225.

    2、Giaccone G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-548.

    3、Yarden Y. et al. (2001) Untangling the ErbB signalling network. Nat Rev Mol Cell Biol. 2(2): 127-137.

  • 稀释度

背景介绍
  • The Epidermal Growth Factor Receptor (EGFR)is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). EGFR is activated after homodimerization or heterodimerization. Heterodimerization is an important mechanism that allows the activation of all EGFR in response to ligand stimulation. The activated EGFR binds to many signaling proteins and stimulates the activation of many signaling pathways. The specificity and potency of intracellular signaling pathways are determined by positive and negative regulators, the specific composition of activating ligand(s), receptor dimer components, and the diverse range of proteins that associate with the tyrosine phosphorylated C-terminal domain of the EGFR.EGFR is overexpressed or mutated in many cancers, especially in breast cancer, ovarian cancer, and non-small cell lung cancer. The overexpression and overactivation of EGFR is correlated with poor prognosis, drug resistance, cancer metastasis, and lower survival rate.

  • 生物活性JSON

    • Immobilized Anti-Human EGFR(Cetuximab) Monoclonal Antibody at 2μg/ml (100μL/well) can bind FITC-Labeled EGFR Fc Chimera, Human with EC50 of 0.19μg/ml.

  • 电泳JSON

    • 2μg (R: reducing conditions, N: non-reducing conditions).