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C5 His Tag Protein, Mouse

C5 His Tag Protein, Mouse

货号: UA010364
价格: 3000
规格: 100μg
介绍: -
其他: -
产品规格

  • 物种

    Mouse

  • 分子别名

    C5a, Complement Component 5, ECLZB, Anaphylatoxin C5a Analog, C5a Anaphylatoxin, C5b, Prepro-C5, CPAMD4, Complement C5, C3 And PZP-Like Alpha-2-Macroglobulin Domain-Containing Protein 4, C5D

  • Accession

    P06684

  • 表达序列

    Gln19-Glu1680, with C-terminal 10*His QEQTYVISAPKILRVGSSENVVIQVHGYTEAFDATLSLKSYPDKKVTFSSGYVNLSPENKFQNAALLTLQPNQVPREESPVSHVYLEVVSKHFSKSKKIPITYNNGILFIHTDKPVYTPDQSVKIRVYSLGDDLKPAKRETVLTFIDPEGSEVDIVEENDYTGIISFPDFKIPSNPKYGVWTIKANYKKDFTTTGTAYFEIKEYVLPRFSVSIELERTFIGYKNFKNFEITVKARYFYNKVVPDAEVYAFFGLREDIKDEEKQMMHKATQAAKLVDGVAQISFDSETAVKELSYNSLEDLNNKYLYIAVTVTESSGGFSEEAEIPGVKYVLSPYTLNLVATPLFVKPGIPFSIKAQVKDSLEQAVGGVPVTLMAQTVDVNQETSDLETKRSITHDTDGVAVFVLNLPSNVTVLKFEIRTDDPELPEENQASKEYEAVAYSSLSQSYIYIAWTENYKPMLVGEYLNIMVTPKSPYIDKITHYNYLILSKGKIVQYGTREKLFSSTYQNINIPVTQNMVPSARLLVYYIVTGEQTAELVADAVWINIEEKCGNQLQVHLSPDEYVYSPGQTVSLDMVTEADSWVALSAVDRAVYKVQGNAKRAMQRVFQALDEKSDLGCGAGGGHDNADVFHLAGLTFLTNANADDSHYRDDSCKEILRSKRNLHLLRQKIEEQAAKYKHSVPKKCCYDGARVNFYETCEERVARVTIGPLCIRAFNECCTIANKIRKESPHKPVQLGRIHIKTLLPVMKADIRSYFPESWLWEIHRVPKRKQLQVTLPDSLTTWEIQGIGISDNGICVADTLKAKVFKEVFLEMNIPYSVVRGEQIQLKGTVYNYMTSGTKFCVKMSAVEGICTSGSSAASLHTSRPSRCVFQRIEGSSSHLVTFTLLPLEIGLHSINFSLETSFGKDILVKTLRVVPEGVKRESYAGVILDPKGIRGIVNRRKEFPYRIPLDLVPKTKVERILSVKGLLVGEFLSTVLSKEGINILTHLPKGSAEAELMSIAPVFYVFHYLEAGNHWNIFYPDTLSKRQSLEKKIKQGVVSVMSYRNADYSYSMWKGASASTWLTAFALRVLGQVAKYVKQDENSICNSLLWLVEKCQLENGSFKENSQYLPIKLQGTLPAEAQEKTLYLTAFSVIGIRKAVDICPTMKIHTALDKADSFLLENTLPSKSTFTLAIVAYALSLGDRTHPRFRLIVSALRKEAFVKGDPPIYRYWRDTLKRPDSSVPSSGTAGMVETTAYALLASLKLKDMNYANPIIKWLSEEQRYGGGFYSTQDTINAIEGLTEYSLLLKQIHLDMDINVAYKHEGDFHKYKVTEKHFLGRPVEVSLNDDLVVSTGYSSGLATVYVKTVVHKISVSEEFCSFYLKIDTQDIEASSHFRLSDSGFKRIIACASYKPSKEESTSGSSHAVMDISLPTGIGANEEDLRALVEGVDQLLTDYQIKDGHVILQLNSIPSRDFLCVRFRIFELFQVGFLNPATFTVYEYHRPDKQCTMIYSISDTRLQKVCEGAACTCVEADCAQLQAEVDLAISADSRKEKACKPETAYAYKVRITSATEENVFVKYTATLLVTYKTGEAADENSEVTFIKKMSCTNANLVKGKQYLIMGKEVLQIKHNFSFKYIYPLDSSTWIEYWPTDTTCPSCQAFVENLNNFAEDLFLNSCEGGGSGGGSHHHHHHHHHH

  • 表达宿主

    HEK293

  • 分子量

    80, 100 & 170kDa

  • 纯度

    >95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1、Ricklin D. et al. (2010) Complement: a key system for immune surveillance and homeostasis. Nat. Immunol. 11: 785-797.

    2、Yan C. et al. (2012) New insights for C5a and C5a receptors in sepsis. Front Immunol. 3: 368.

  • 稀释度

背景介绍
  • The complement system is an important component of the innate immunity that functions Primarily as a first-line host defense against pathogenetic infections.C5a is one of the complement family protein. During complement activation, C3 and C5 are cleaved, releasing small soluble fragments known as C3a and C5a, respectively.C5a and C3a are anaphylatoxins, which are potent proinflammatory molecules that attract and activate leukocytes through interaction with their cognate G-protein–coupled receptors, C3a receptor (C3aR) and C5a receptor (C5aR). C5 is composed of alpha and beta polypeptide chains with a disulfide bridge and is cleaved by C5 convertases to produce C5a and C5b.C5a is an anaphylatoxin that induces chemotactic activity.C5a can also activate neutrophils that release inflammatory cytokines such as IL-8.C5b forms a complex with complement C6 and other complement components to produce C5b-9, a membrane attack complex (MAC) that eventually disrupts the cell membrane of target cells.C5a, the most potent plasma-derived chemotactic factor, is a 74-amino-acid peptide/protein released from the α-chain of C5 by either the classical or alternative pathway C5 convertases. Structurally, C5a consists of four antiparallel α-helices held together by disulfide bonds and an 11-amino-acid carboxy terminal tail. The α-helical bundle interacts with the receptor's amino-terminal domain, whereas the tail appears to insert itself into the transmembrane bundle of the receptor, thereby activating the receptor.C5a is a strong chemoattractant and is involved in the recruitment of inflammatory cells such as neutrophils, eosinophils, monocytes, and T lymphocytes, in activation of phagocytic cells and release of granule-based enzymes and generation of oxidants, all of which may contribute to innate immune functions or tissue damage.C5a provides a vital bridge between innate and adaptive immune functions, extending the roles of C5a in inflammation.

  • 电泳JSON

    • 1μg (R: reducing condition, N: non-reducing condition).