Val 114-Gly281
19-20kDa (Reducing)
＞95% by SDS-PAGE & RP-HPLC
20mM Tris, 150mM NaCl, 1mM TCEP, pH8.0
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), also known as CD253 or TNFSF10, can be produced and secreted by most normal tissue cells. TRAIL proteins are available in two forms: membrane-bound (full-length TRAIL) and soluble (sTRAIL). In vivo, only the membrane-bound form exists, while the soluble polypeptide (sTRAIL), which is formed in the extracellular region of TRAIL, has the full biological activity of the membrane-bound TRAIL. Both of them are able to bind to specific receptors on the cell membrane and rapidly induce the expression of TRAIL through intracellular signal transduction. They can rapidly induce apoptosis in cells expressing TRAIL-specific receptors. TRAIL has a broad spectrum of action and can induce apoptosis in a variety of tumor cells in the colon, lung, breast, kidney, brain and skin, and is considered as a promising broad-spectrum anti-cancer molecule.
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D，The EC50 for this effect is less than 0.8ng/ml.
Immobilized TRAIL/Apo2L, Human (Cat. No. UA040039) at 2.0μg/mL (100μL/well) can bind TRAIL R1/TNFRSF10A Fc Chimera, Human (Cat. No. UA010630) with EC50 of 3.51-5.40 ng/mL.
Anti-His antibody Immobilized on CM5 Chip captured TNFRSF10B/TRAIL R2 His Tag, Mouse (Cat. No. UA010509), can bind TRAIL/Apo2L, Human (Cat. No. UA040039) with an affinity constant of 4.67nM as determined in SPR assay.
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