您现在的位置： 首页   -  产品中心   -  免疫学   -   抗体
IL-6R alpha/CD126 Fc Chimera Protein, Human

IL-6R alpha/CD126 Fc Chimera Protein, Human

货号: UA010448
价格: 2970
规格: 100μg
介绍: -
其他: -
产品规格

  • 物种

    Human

  • 抗原名称

    IL-6R alpha/CD126

  • 分子别名

    IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R-alpha, IL-6RA

  • Accession

    P08887

  • 表达序列

    Leu20 - Pro365, with C-terminal Human IgG1 Fc

    LAPRRCPAQEVARGVLTSLPGDSVTLTCPGVEPEDNATVHWVLRKPAAGSHPSRWAGMGRRLLLRSVQLHDSGNYSCYRAGRPAGTVHLLVDVPPEEPQLSCFRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQNSPAEDFQEPCQYSQESQKFSCQLAVPEGDSSFYIVSMCVASSVGSKFSKTQTFQGCGILQPDPPANITVTAVARNPRWLSVTWQDPHSWNSSFYRLRFELRYRAERSKTFTTWMVKDLQHHCVIHDAWSGLRHVVQLRAQEEFGQGEWSEWSPEAMGTPWTESRSPPAENEVSTPMQALTTNKDDDNILFRDSANATSLPVQDSSSVPLPIEGRMDPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

  • 表达宿主

    HEK293

  • 分子量

    76-93 kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    Human Fc Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.M Hibi, M Murakami, M Saito, T Hirano, T Taga, TKishimoto. Molecular cloning and expression of an IL-6 signal transducer,gp130.Cell. 1990 Dec 21;63(6):1149-57.
    2. Bin S, Xin L, Lin Z, Jinhua Z, Rui G, Xiang Z.Targeting miR-10a-5p/IL-6Raxis for reducing IL-6-induced cartilage cell ferroptosis.Exp Mol Pathol. 2021Feb;118:104570. Cell., 63 (1990), pp. 1149-1157.

  • 稀释度

背景介绍

  • The Interleukin-6 receptor (IL-6R) consists of an 80-kDa IL-6 binding protein (α chain) (CD126) and gp130. The alpha-chain CD126 is a glycoprotein that contains the ligand-binding site. The human IL-6R protein contains 468 amino acids, which includes a signal peptide, an extracellular region, a transmembrane domain, and a short cytoplasmic domain of 19, 339, 28, and 82 amino acids, respectively. IL-6R are present on several types of tumor cells, including multiple myeloma, hepatocellular carcinoma, prostate carcinoma , and epidermoid carcinoma. The IL-6/IL-6R signal pathway promotes the tumor growth in various cancers, especially for glioma. Hence, IL-6R not only can be used as a target site for transporting drugs, but also as a therapeutic site. Primary human cartilage cells express IL-6R, which is further induced in the presence of IL-6. This makes cartilage cells direct targets or effectors of IL-6.

  • 生物活性JSON

    • Protein A Chip captured IL-6R alpha/CD126 Fc Chimera, Human (Cat. No. UA010448), can bind IL-6, Human (Cat. No. UA040052) with an affinity constant of 8.51 nM as determined in SPR assay.

  • 电泳JSON

    • 2μg (R: reducing conditions, N: non-reducing conditions).

  • ELISA

    • Immobilized IL-6, Human (Cat. No. UA040052) at 5.0μg/mL (100μL/well) can bind IL-6R alpha/CD126 Fc Chimera, Human (Cat. No. UA010448) with EC50 of 3.04-3.92ng/ml.