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Biotinylated MSLN Fc&Avi Tag Protein, Human

Biotinylated MSLN Fc&Avi Tag Protein, Human

货号: UA010459
价格: 2120
规格: 25μg
介绍: -
其他: -
产品规格

  • 物种

    Human

  • 抗原名称

    MSLN

  • 分子别名

    CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

  • Accession

    Q13421-1

  • 表达序列

    Glu296-Gly580, with C-terminal Human IgG1 Fc & Avi tag

    EVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQAPRRPLPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDIEGRMDPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKGLNDIFEAQKIEWHE

  • 表达宿主

    HEK293

  • 分子量

    72-85kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Biotin

  • 标签

    Human Fc Tag, Avi Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.Hassan, R. et al: Mesothelin: a new target for immunotherapy, Clin.Cancer Res. 10:3937(2004).

  • 稀释度

背景介绍

  • Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF. MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation. The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

  • 电泳JSON

    • 1μg (R: reducing conditions, N: non-reducing conditions).

  • ELISA

    • Immobilized Anti-Human MSLN Monoclonal Antibody (Amatuximab) at 2.0μg/mL (100μL/well) can bind Biotinylated MSLN Fc&Avi Tag, Human (Cat. No. UA010459)with EC50 of 4.86-5.99ng/mL.