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GFP His Tag protein, Aequorea victoria

GFP His Tag protein, Aequorea victoria

货号: UA070020
价格: 1320
规格: 50μg
介绍: -
其他: -
产品规格
  • 物种

    Aequorea victoria
  • 抗原名称

    GFP
  • 分子别名

    GFP Protein
  • Accession

    AAB65663
  • 表达序列

    Ser2-Lys238, with N-terminal 8*His HHHHHHHHSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK

  • 表达宿主

    E.coli
  • 分子量

    27.8kDa
  • 纯度

    >95% by SDS-PAGE&RP-HPLC
  • 内毒素含量

    <0.1EU/μg
  • 标记

    Unconjugated
  • 标签

    His Tag
  • 性状

    Lyophilized Powder
  • 缓冲体系

    PBS, pH7.4
  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
  • 储存条件

    12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.Trends Biochem Sci 1995 Nov;20(11):448-55.
    2.Biol Chem. 1998 Dec 25;273(52):34970-5.

  • 稀释度

背景介绍
  • The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. It is a widely used reporter in gene expression and protein localization studies. the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is a widely used reporter in studies of gene expression and protein localization. GFP is a single chain polypeptide of 238 amino acids .Most of these amino acids form β sheets that are compacted through an antiparallel structure to form the barrel. So far, they have been used as reporters of gene expression, tracers of cell lineage, and as fusion tags to monitor protein localization within living cells.

  • 电泳JSON

    • 1μg (R: reducing conditions, N: non-reducing conditions).

  • 反相高效液相色谱JSON(RP-HPLC)